Canine Cytochrome P450scc side chain cleavage Enzyme ELISA Kit from MyBioSource.com

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Canine Cytochrome P450scc side chain cleavage Enzyme ELISA Kit

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Description

Principle of the Assay: P450scc ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P450scc antibody and an P450scc-HRP conjugate. The assay sample and buffer are incubated together with P450scc-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P450scc concentration since P450scc from samples and P450scc-HRP conjugate compete for the anti-P450scc antibody binding site. Since the number of sites is limited, as more sites are occupied by P450scc from the sample, fewer sites are left to bind P450scc-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P450scc concentration in each sample is interpolated from this standard curve